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A compact CMOS biochip immunosensor towards the detection of a single bacteria by Joon Myong Song; Mustafa Culha; Paul M. Kasili; Guy D. Griffin; Tuan Vo-Dinh is a Engineering article available to read on EtoBox.
What is A compact CMOS biochip immunosensor towards the detection of a single bacteria about?
Recent use of biological warfare (BW) agents has led to a growing interest in the rapid and sensitive detection of pathogens. Therefore, the development of field-usable detection devices for sensitive and selective detection of BW agents is an important issue. In this work, we report a portable biochip system based on complementary metal oxide semiconductor (CMOS) technology that has great potential as a device for single-bacteria detection. The possibility of single-bacteria detection is reported using an immunoassay coupled to laser-induced fluorescence (LIF) detection. Bacillus globigii spores, which are a surrogate species for B. anthracis spores, were used as the test sample. Enzymatic amplification following immunocomplex formation allowed remarkably sensitive detection of B. globigii spores, and could preclude a complicated optical and instrumental system usually required for high-sensitive detection. Atomic force microscopy (AFM) was employed to investigate whether B. globigii spores detected in the portable biochip system exist in single-cell or multicellular form. It was found that B. globigii spores mostly exist in multicellular form with a small minority of single-cell
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- Author
- Joon Myong Song; Mustafa Culha; Paul M. Kasili; Guy D. Griffin; Tuan Vo-Dinh
- Publisher
- Elsevier Science; Elsevier ; Elsevier Ltd.; Elsevier BV (ISSN 0956-5663)
- Published
- 2005
- Language
- EN
- Field
- Engineering (Physical Sciences)