Can I read Determination of testosterone metabolites in human hepatocytes: I. Development of an on-line sample preparation liquid chromatography technique and mass spectroscopic detection of 6β-hydroxytestosterone on EtoBox?

Determination of testosterone metabolites in human hepatocytes: I. Development of an on-line sample preparation liquid chromatography technique and mass spectroscopic detection of 6β-hydroxytestosterone by Gerhard Friedrich; Thorsten Rose; Klaus Rissler is a Chemistry article available to read on EtoBox.

What is Determination of testosterone metabolites in human hepatocytes: I. Development of an on-line sample preparation liquid chromatography technique and mass spectroscopic detection of 6β-hydroxytestosterone about?

A rapid and sensitive RP-HPLC assay for determination of 6beta-hydroxytestosterone in human hepatocytes with corticosterone as the internal standard is described. The procedure employs on-line sample enrichment using a BioTrap 500 MS (20x4 mm I.D.) extraction pre-column and subsequent gradient separation on a Prontosil 60-5 C(18)-H (250x2 mm I.D., 5 micrometer particle size) analytical column in the back-flush mode using a ternary eluent system composed of methanol, tetrahydrofuran and water. Signal monitoring was done by measurement of the responses from liquid chromatography coupled to mass spectroscopy (LC-MS/MS) using an atmospheric pressure chemical ionization (APCI) source conducted in the selected reaction monitoring (SRM) mode. Mean recoveries of 6beta-hydroxytestosterone from an estimate of the biological matrix, i.e., Dulbecco's modified Eagle medium "High Glucose", ranged from 101.8-104.4% for samples containing the target analyte at the 250, 500 and 1000 ng/ml level. The limit of quantitation (LOQ) was 20 ng/ml at an injection volume of 100 microliter determined in the same matrix. Linearity of signal responses versus concentration for all three analytes was accomplishe

Who reads Determination of testosterone metabolites in human hepatocytes: I. Development of an on-line sample preparation liquid chromatography technique and mass spectroscopic detection of 6β-hydroxytestosterone?

Author

Gerhard Friedrich; Thorsten Rose; Klaus Rissler

Publisher

Elsevier Science; Elsevier ; Elsevier BV (ISSN 1873-376X)

Published

2003

Language

EN

Field

Chemistry (Physical Sciences)