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Can I read Immunodetection of caspase-3 by Western blot using glutaraldehyde on EtoBox?
Immunodetection of caspase-3 by Western blot using glutaraldehyde by Gabriela Nestal de Moraes; Érika Carvalho; Raquel C. Maia; Cinthya Sternberg is a Biochemistry, Genetics and Molecular Biology article available to read on EtoBox.
What is Immunodetection of caspase-3 by Western blot using glutaraldehyde about?
Cell death by apoptosis triggers the engagement of a conserved intracellular machinery of execution, involving mainly the activation of the caspase family of cysteine proteases. Caspase-3 is a common effector of most of the apoptotic pathways and is able to cleave several target proteins whose degradation will contribute to the execution phase of the cell demise program. Here we present a modification of the Western blot protocol to improve sensitivity of caspase-3 detection, providing a valuable tool to access its activation in biological specimens.
Who reads Immunodetection of caspase-3 by Western blot using glutaraldehyde?
It is typically read by researchers, students, and practitioners in Biochemistry, Genetics and Molecular Biology.
- Author
- Gabriela Nestal de Moraes; Érika Carvalho; Raquel C. Maia; Cinthya Sternberg
- Publisher
- Elsevier Science; Elsevier ; Elsevier Inc.; Elsevier BV (ISSN 0003-2697)
- Published
- 2011
- Language
- EN
- Field
- Biochemistry, Genetics and Molecular Biology (Life Sciences)