About this document
PBabe Retrovirus Production by wc9663 is a document available to read on EtoBox.
This document outlines the protocol for producing pBabe retrovirus using 293.T cells, detailing the transfection process, media changes, and viral supernatant harvesting. Key steps include mixing specific plasmids, adding transfection reagents, and ultracentrifugation to concentrate the virus. The final steps involve resuspending the virus and storing it properly to maintain titer stability.
- Author
- wc9663
- Language
- EN