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The fidelity of spin trapping with DMPO in biological systems by Kalina Ranguelova; Ronald P. Mason is a Chemistry article available to read on EtoBox.
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## Abstract Unlike direct ESR, spin trap methodology depends on the absolute fidelity of the spin trap reaction. Two alternative reactions of 5,5‐dimethyl‐1‐pyrroline __N__‐oxide (DMPO) leading to radical adduct artifacts have been discovered and investigated: inverted spin trapping and the Forrester–Hepburn nucleophilic mechanism. These two alternate pathways to radical adducts are a combination of one‐electron oxidation and nucleophilic addition, in either order. In biological systems, serious artifacts have been reported due to the Forrester–Hepburn mechanism, which is initiated by the addition of a nucleophile to DMPO. It has recently been demonstrated that (bi)sulfite (hydrated sulfur dioxide) can react with DMPO via a nonradical, nucleophilic reaction, and it has been further proposed that DMPO/^•^SO~3~^−^ formation in biological systems is an artifact and not the result of spin trapping of sulfur trioxide anion radical (^•^SO~3~^−^). The one‐electron oxidation of (bi)sulfite catalyzed by horseradish peroxidase (HRP)/hydrogen peroxide (H~2~O~2~) has been reinvestigated by ESR spin trapping with DMPO and oxygen uptake studies to obtain further evidence for the radical reaction
Who reads The fidelity of spin trapping with DMPO in biological systems?
It is typically read by researchers, students, and practitioners in Chemistry.
- Author
- Kalina Ranguelova; Ronald P. Mason
- Publisher
- John Wiley and Sons; Wiley (John Wiley & Sons); John Wiley & Sons Inc.; John Wiley and Sons Ltd; Wiley (ISSN 0749-1581)
- Published
- 2011
- Language
- EN
- Field
- Chemistry (Physical Sciences)