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Design, one-pot synthesis, cytotoxic, in vivo anticancer, antioxidant and antimicrobial evaluation of a novel mixed schiff ... 1 1 Introduction 1 2 Experimental 2 2.1 Instrumental methods 2 2.2 Synthesis 2 2.2.1 Synthesis of Schiff base ligand 2 2.2.2 Ligand, 3-((2-((1-(2-hydroxyphenyl)ethylidene)amino)ethyl)imino)-N-(o-tolyl)butanamide 2 2.3 Metal complexes, general procedure 2 2.3.1 Synthesis of [Mn(II)L(H2O)2] 2 2.3.2 Synthesis of [Fe(III)L(H2O)Cl] 3 2.3.3 Synthesis of [Co(II)L] 3 2.3.4 Synthesis of [Ni(II)L] 3 2.3.5 Synthesis of [Cu(II)L] 3 2.4 Anticancer studies 3 2.4.1 Preparation of the drug 3 2.4.2 Percentage of cytotoxicity by Trypan blue exclusion method 4 2.4.3 Methyl tetrazolium assay (MTT Assay) 4 2.4.4 Procedure for in vivo toxicity studies of copper complex in mice 4 2.4.4.1 Treatment of Cu(II) complex against EAC induced tumor in mice and their survival rate 4 2.4.4.2 Treatment Cu(II) complex against DLA induced solid tumour 5 2.5 Antioxidant assays 5 2.6 Antimicrobial studies 5 2.6.1 Minimum inhibitory concentration 5 3 Results and discussions 5 3.1 Characterization of the ligand, H2L 5 3.1.1 1H NMR spectrum 5 3.1.1.1 IR spectrum 6 3.1.1.2 The UV-visible spectrum

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nonfiction