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What is Affinity Chromatography Overview and Methods about?

The document summarizes the history and process of affinity chromatography. It dates back to 1910 but was first published and demonstrated in 1967 and 1978, respectively. Affinity chromatography involves the interaction of a ligand with the solute of interest in a manner comparable to ion exchange. Key components are the ligand, spacer, and matrix which are used to purify samples like enzymes, antibodies, hormones through biospecific or nonspecific elution methods.

Author

Radhika Konakalla

Language

EN