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Isolation of two soybean G‐box binding factors which interact with a G‐box sequence of an auxin‐responsive gene by Jong Chan Hong; Yong Hwa Cheong; Ron T. Nagao; Jeong Dong Bahk; Joe L. Key; Moo Je Cho is a Agricultural and Biological Sciences article available to read on EtoBox.

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G‐box binding factors (GBFs) constitute a family of plant DNA‐binding proteins that bind to the G‐box motif, a regulatory __cis__ element present in many plant genes with a palindromic DNA motif of CACGTG. Previously TCCACGTGTC, a G‐box motif, from an auxin responsive gene __GmAux28__ has been identified as a sequence‐specific protein‐binding site. Here the isolation of two soybean cDNA clones, referred to as SGBF‐1 and SGBF‐2, encoding proteins which bind to the G‐box motif is reported. The primary structure of SGBF‐1 and SGBF‐2 predicts that these proteins contain a basic leucine zipper (bZIP) DNA‐binding domain and an N‐terminal proline‐rich domain. A dramatic difference in the pattern of protein‐DNA complex formation was observed when recombinant SGBF‐1 and SGBF‐2 proteins were analyzed by electrophoretic mobility shift assays (EMSAs). The SGBF‐1 binding pattern obtained with the G‐box probe resulted in three major retarded bands while the SGBF‐2 formed a single complex. This shows that the characteristically diffuse banding pattern of plant nuclear proteins interacting with the G‐box is also observed in a binding assay using only one recombinant GBF. EMSAs were performed with

Who reads Isolation of two soybean G‐box binding factors which interact with a G‐box sequence of an auxin‐responsive gene?

Author

Jong Chan Hong; Yong Hwa Cheong; Ron T. Nagao; Jeong Dong Bahk; Joe L. Key; Moo Je Cho

Publisher

John Wiley and Sons; Wiley (Blackwell Publishing); Blackwell Publishing Inc.; Wiley (ISSN 0960-7412)

Published

1995

Language

EN

Field

Agricultural and Biological Sciences (Life Sciences)