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Catenary Cultures of Embryonic Gastrointestinal Tract Support Organ Morphogenesis, Motility, Neural Crest Cell Migration, and Cell Differentiation by Catherine J. Hearn; Heather M. Young; Daniela Ciampoli; Alan E.G. Lomax; Don Newgreen is a Biochemistry, Genetics and Molecular Biology article available to read on EtoBox.
What is Catenary Cultures of Embryonic Gastrointestinal Tract Support Organ Morphogenesis, Motility, Neural Crest Cell Migration, and Cell Differentiation about?
The embryonic gastrointestinal tract develops from a simple tube into a coiled, flexed, and regionalized structure. The changes in gut morphology coincide with the differentiation of multiple cell types in concentric layers, and include colonization by migratory neuron precursors, and the development of gastrointestinal motility. We describe a reliable method for growing embryonic mouse intestine in vitro by the attachment of segments of intestinal tract by their cut ends, with the intervening region suspended in the culture medium. These are termed ''catenary cultures.'' E11-E11.5 mouse midgut, hindgut, or mid-plus hindgut segments were grown in catenary culture for up to 10 days and their growth, morphology, cell differentiation, ability to support neural precursor migration, and contractile activity were assessed. The increase in size of the cultured explants was not large, but morphogenesis proceeded, best exemplified by elongation of the caecum. Cell differentiation also proceeded. In the mucosa, goblet cells differentiated. Muscle layers, characterized by desmin expression, and kit-positive interstitial cells of Cajal differentiated in the correct positions. Where segments in
Who reads Catenary Cultures of Embryonic Gastrointestinal Tract Support Organ Morphogenesis, Motility, Neural Crest Cell Migration, and Cell Differentiation?
It is typically read by researchers, students, and practitioners in Biochemistry, Genetics and Molecular Biology.
- Author
- Catherine J. Hearn; Heather M. Young; Daniela Ciampoli; Alan E.G. Lomax; Don Newgreen
- Publisher
- John Wiley and Sons; Wiley (John Wiley & Sons); John Wiley & Sons Inc.; Wiley (ISSN 1058-8388)
- Published
- 1999
- Language
- EN
- Field
- Biochemistry, Genetics and Molecular Biology (Life Sciences)