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Can I read Comparison of Surgical and Cadaveric Intestine as a Source of Crypt Culture in Humans on EtoBox?
Comparison of Surgical and Cadaveric Intestine as a Source of Crypt Culture in Humans by Andrew Scott; Barbara Olack; Joshua D. Rouch; Hassan A. Khalil; Brent A. Kokubun; Nan Ye Lei; Jiafang Wang; Sergio Solorzano; Michael Lewis; James C.Y. Dunn; Matthias G. Stelzner; Joyce C. Niland; Martín G. Martín is a Medicine article available to read on EtoBox.
What is Comparison of Surgical and Cadaveric Intestine as a Source of Crypt Culture in Humans about?
Human small intestinal crypts are the source of intestinal stem cells (ISCs) that are capable of undergoing self-renewal and differentiation to an epithelial layer. The development of methods to expand the ISCs has provided opportunities to model human intestinal epithelial disorders. Human crypt samples are usually obtained from either endoscopic or discarded surgical samples, and are thereby exposed to warm ischemia, which may impair their __in vitro__ growth as three-dimensional culture as spheroids or enteroids. In this study we compared duodenal samples obtained from discarded surgical samples to those isolated from whole-body preserved cadaveric donors to generate __in vitro__ cultures. We also examined the effect of storage solution (phosphate-buffered saline or University of Wisconsin [UW] solution) as well as multiple storage times on crypt isolation and growth in culture. We found that intestinal crypts were successfully isolated from cadaveric tissue stored for up to 144 h post-procurement and also were able to generate enteroids and spheroids in certain media conditions. Surgical samples stored in UW after procurement were sufficiently viable up to 24 h and also allowed
Who reads Comparison of Surgical and Cadaveric Intestine as a Source of Crypt Culture in Humans?
It is typically read by researchers, students, and practitioners in Medicine.
- Author
- Andrew Scott; Barbara Olack; Joshua D. Rouch; Hassan A. Khalil; Brent A. Kokubun; Nan Ye Lei; Jiafang Wang; Sergio Solorzano; Michael Lewis; James C.Y. Dunn; Matthias G. Stelzner; Joyce C. Niland; Martín G. Martín
- Published
- 2020
- Language
- EN
- Field
- Medicine (Health Sciences)