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PCR‐based detection of sterol demethylation inhibitor‐resistant strains of Penicillium digitatum by Hiroshi Hamamoto; Koji Hasegawa; Ryoji Nakaune; Young Jin Lee; Katsumi Akutsu; Tadaaki Hibi is a Agricultural and Biological Sciences article available to read on EtoBox.

What is PCR‐based detection of sterol demethylation inhibitor‐resistant strains of Penicillium digitatum about?

## Abstract A simple method for detecting sterol demethylation inhibitor (DMI)‐resistant strains of the citrus green mould pathogen, __Penicillium digitatum__, has been developed. The method involves detection of a tandem repeat of a transcriptional enhancer in the promoter region of __PdCYP51__, which encodes the target enzyme of DMIs, by PCR, using conidia as template. The presence of the tandem repeat leads to overexpression of this gene and confers DMI resistance to the fungus. We examined the relationship between the presence of the tandem repeat and DMI resistance in 39 strains of __P digitatum__. The results suggested that the DMI resistance mechanism based on the tandem repeat is common in this fungus. Using this method, the presence of DMI resistance of this fungus can be detected within 5–6 h. © 2001 Society of Chemical Industry

Who reads PCR‐based detection of sterol demethylation inhibitor‐resistant strains of Penicillium digitatum?

Author

Hiroshi Hamamoto; Koji Hasegawa; Ryoji Nakaune; Young Jin Lee; Katsumi Akutsu; Tadaaki Hibi

Publisher

John Wiley and Sons; Wiley (John Wiley & Sons); John Wiley & Sons Inc.; Wiley (ISSN 1526-498X)

Published

2001

Language

EN

Field

Agricultural and Biological Sciences (Life Sciences)