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Expression of Wnt and Notch pathway genes in a pluripotent human embryonal carcinoma cell line and embryonic stem cells by JAMES WALSH; PETER W. ANDREWS is a Medicine article available to read on EtoBox.

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Embryonal carcinoma (C) cells, the pluripotent stem cells of teratocarcinomas, show many similarities to embryonic stem (S) cells. Since EC cells are malignant but their terminally differentiated derivatives are not, understanding the molecular mechanisms that regulate their differentiation may be of value for diagnostic and therapeutic purposes. We have examined the expression of multiple components of two developmentally important cell‐cell signalling pathways, Wnt and Notch, in the pluripotent human EC cell line, NTERA2, and the human ES cell line, H7. Both pathways have well‐documented roles in controlling neurogenesis, a process that occurs largely in response to retinoic acid (A) treatment of NTERA2 cultures and spontaneously in H7 cultures. In NTERA2, many of the genes tested showed altered transcriptional regulation following treatment with RA. These include members of the __frizzled__ gene family (__FZD1__, __FZD3__, __FZD4__, __FZD5__, __FZD6__), encoding receptors for Wnt proteins, the __Frizzled____Related Protein__ family (__SFRP1__, __SFRP2__, __FRZB__, __SFRP4__), encoding soluble Wnt antagonists and also ligands and receptors of the Notch pathway (__Dlk1__, __Jagged

Who reads Expression of Wnt and Notch pathway genes in a pluripotent human embryonal carcinoma cell line and embryonic stem cells?

Author

JAMES WALSH; PETER W. ANDREWS

Publisher

John Wiley and Sons; Wiley (Blackwell Publishing); Blackwell Publishing Inc.; Wiley (ISSN 0365-5555)

Published

2003

Language

EN

Field

Medicine (Health Sciences)