Can I read Temporal Dependence of Ectopeptidase Expression in Alveolar Epithelial Cell Culture: Implications for Study of Peptide Absorption on EtoBox?

Temporal Dependence of Ectopeptidase Expression in Alveolar Epithelial Cell Culture: Implications for Study of Peptide Absorption by B Forbes; C.G Wilson; M Gumbleton is a Pharmacology, Toxicology and Pharmaceutics article available to read on EtoBox.

What is Temporal Dependence of Ectopeptidase Expression in Alveolar Epithelial Cell Culture: Implications for Study of Peptide Absorption about?

There is little data available regarding the extent of peptide metabolism encountered following inhalation to the lung. We have studied the activity of five ectopeptidases in primary rat alveolar epithelial cells, A549 cells and pulmonary macrophages. Peptidase activity was assayed in the plasma membrane fractions (PMF) of primary type II alveolar epithelial cells (ATII cells) after 2 days in culture and after 7 days in culture when they had formed monolayers of type I-like cells (ATI-like cells). Dipeptidyl peptidase IV (DPP) activity fell from 36.65 mU/mg protein to 16.29 mU/mg protein between day 2 and day 7 in culture, aminopeptidase N (AMN) activity increased from 16.16 mU/mg protein to 23.99 mU/mg protein, angiotensin-converting enzyme (ACE) activity was lost (4.29 mU/mg protein at day 2, not detected at day 7), and carboxypeptidase M (CPM) activity was acquired (not detected at day 2, 5.20 mU/mg protein at day 7). The profile of exopeptidase expression in A549 cells was similar to that of primary rat alveolar cells at day 7 in culture (DPP 24.24 mU/mg protein, AMN 47.74 mU/mg protein, CPM 4.28 mU/mg protein, ACE not detected). Macrophages expressed high levels of aminopeptid

Who reads Temporal Dependence of Ectopeptidase Expression in Alveolar Epithelial Cell Culture: Implications for Study of Peptide Absorption?

Author

B Forbes; C.G Wilson; M Gumbleton

Publisher

Elsevier Science; Elsevier ; Elsevier BV (ISSN 0378-5173)

Published

1999

Language

EN

Field

Pharmacology, Toxicology and Pharmaceutics (Life Sciences)