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Can I read Immortalization without neoplastic transformation of human mesenchymal stem cells by transduction with HPV16 E6/E7 genes on EtoBox?
Immortalization without neoplastic transformation of human mesenchymal stem cells by transduction with HPV16 E6/E7 genes by Shih‐Chieh Hung; Den‐Mei Yang; Ching‐Fang Chang; Ruey‐Jen Lin; Jih‐Shiuan Wang; Larry Low‐Tone Ho; Wen K. Yang is a Medicine article available to read on EtoBox.
What is Immortalization without neoplastic transformation of human mesenchymal stem cells by transduction with HPV16 E6/E7 genes about?
## Abstract hMSCs derived from bone marrow are useful as a species‐specific cell culture system for studying cell lineage differentiation and tissue remodeling. However, hMSCs usually have a short __in vitro__ life span due to replicative senescence. We therefore used a high dose of retroviral vector LXSN‐16E6E7 to transduce hMSCs of an aging donor and obtained an actively proliferating cell line, designated KP‐hMSCs, which expressed HPV16 E6/E7 mRNA. Whereas parental hMSCs ceased to grow after 30 PDs, KP‐hMSCs could be propagated beyond 100 PDs. With culture procedures to avoid selection pressure and crowded cell growth, KP‐hMSCs showed no signs of neoplastic transformation as examined by soft‐agar anchorage‐independent growth and NOD‐SCID mouse tumorigenicity assays. KP‐hMSCs gave similar cytofluorimetric profiles of 31 CD markers to those of the parental primary hMSCs, except with some morphologic changes and expansion of an originally very minor CD34^dim^CD38^+^CD50^+^ cell population. Upon exposure to specific stimulating conditions __in vitro__, KP‐hMSCs could respond and differentiate along the mesenchymal (bone, fat and cartilage) and nonmesenchymal (neuron) cell lineages.
Who reads Immortalization without neoplastic transformation of human mesenchymal stem cells by transduction with HPV16 E6/E7 genes?
It is typically read by researchers, students, and practitioners in Medicine.
- Author
- Shih‐Chieh Hung; Den‐Mei Yang; Ching‐Fang Chang; Ruey‐Jen Lin; Jih‐Shiuan Wang; Larry Low‐Tone Ho; Wen K. Yang
- Publisher
- John Wiley and Sons; Wiley (John Wiley & Sons); John Wiley & Sons Inc.; Wiley; Research Square; Test accounts (ISSN 0020-7136)
- Published
- 2004
- Language
- EN
- Field
- Medicine (Health Sciences)