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Bifunctional Electro-optical Nanoprobe to Real-time Detect Local Biochemical Processes in Single Cells by Xin Ting Zheng; Weihua Hu; Houxiao Wang; Hongbin Yang; Wei Zhou; Chang Ming Li is a Engineering article available to read on EtoBox.

What is Bifunctional Electro-optical Nanoprobe to Real-time Detect Local Biochemical Processes in Single Cells about?

A bifunctional electro-optical nanoprobe with integrated nanoring electrode and optical nanotip was fabricated and investigated to simultaneously detect both electrical and optical signals in real-time with high spatial resolution. Concurrent measurements of the oxidant generation and the intracellular antioxidant levels in single cells correlate the stronger oxidant generation with an altered initial antioxidant response in the breast cancer cells in comparison to the normal ones suggesting that the cell malignancy is associated with the strength of oxidative stress, and the higher antioxidant level may be the cause of the drug resistance. While the optical detection indicates the fluctuation of the intracellular redox homeostasis, the chronoamperometric signals allow quantitative real-time detection of the H 2 O 2 release and decay. Furthermore, the nanoscale probe enables localized simultaneous detections thus discovering that activated enzymes responsible for the oxidative stress target at specific membrane regions. This method promises applications in study of the dynamics of important physiological processes, and provides the opportunity to unravel the interplay of various si

Who reads Bifunctional Electro-optical Nanoprobe to Real-time Detect Local Biochemical Processes in Single Cells?

Author

Xin Ting Zheng; Weihua Hu; Houxiao Wang; Hongbin Yang; Wei Zhou; Chang Ming Li

Publisher

Elsevier Science; Elsevier ; Elsevier Ltd.; Elsevier BV (ISSN 0956-5663)

Published

2011

Language

EN

Field

Engineering (Physical Sciences)