About this Engineering article
An Ionizable Lipid Material with a Vitamin E Scaffold as an mRNA Vaccine Platform for Efficient Cytotoxic T Cell Responses by Ryotaro Oyama; Harumichi Ishigame; Hiroki Tanaka; Naho Tateshita; Moeko Itazawa; Ryosuke Imai; Naomasa Nishiumi; Jun-ichi Kishikawa; Takayuki Kato; Jessica Anindita; Yoshifumi Nishikawa; Masatoshi Maeki; Manabu Tokeshi; Kota Tange; Yuta Nakai; Yu Sakurai; Takaharu Okada; Hidetaka Akita is a Engineering article available to read on EtoBox.
RNA vaccines based on lipid nanoparticles (LNPs) with __in vitro__ transcribed mRNA (IVT-mRNA) encapsulated are now a currently successful but still evolving modality of vaccines. One of the advantages of RNA vaccines is their ability to induce CD8^+^ T-cell-mediated cellular immunity that is indispensable for excluding pathogen-infected cells or cancer cells from the body. In this study, we report on the development of LNPs with an enhanced capability for inducing cellular immunity by using an ionizable lipid with a vitamin E scaffold. An RNA vaccine that contained this ionizable lipid and an IVT-mRNA encoding a model antigen ovalbumin (OVA) induced OVA-specific cytotoxic T cell responses and showed an antitumor effect against an E.G7-OVA tumor model. Vaccination with the LNPs conferred protection against lethal infection by __Toxoplasma gondii__ using its antigen TgPF. The vitamin E scaffold-dependent type I interferon response was important for effector CD8^+^ T cell differentiation induced by the mRNA-LNPs. Our findings also revealed that conventional dendritic cells (cDCs) were essential for achieving CD8^+^ T cell responses induced by the mRNA-LNPs, while the XCR1-positive su
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- Author
- Ryotaro Oyama; Harumichi Ishigame; Hiroki Tanaka; Naho Tateshita; Moeko Itazawa; Ryosuke Imai; Naomasa Nishiumi; Jun-ichi Kishikawa; Takayuki Kato; Jessica Anindita; Yoshifumi Nishikawa; Masatoshi Maeki; Manabu Tokeshi; Kota Tange; Yuta Nakai; Yu Sakurai; Takaharu Okada; Hidetaka Akita
- Publisher
- American Chemical Society (ACS)
- Published
- 2023
- Language
- EN
- Field
- Engineering (Physical Sciences)