About this Biochemistry, Genetics and Molecular Biology article

CD44v3,8–10 is involved in cytoskeleton-mediated tumor cell migration and matrix metalloproteinase (MMP-9) association in metastatic breast cancer cells by Lilly Y. W. Bourguignon; Zeenat Gunja-Smith; Naoko Iida; H. B. Zhu; L. J. T. Young; William J. Muller; R. D. Cardiff is a Biochemistry, Genetics and Molecular Biology article available to read on EtoBox.

In the present study, we have employed a unique breast cancer cell line (Met-1, which was derived from a high metastatic potential tumor in transgenic mice expressing polyomavirus middle T oncogene) to study the role of CD44 variant isoform(s) in the regulation of metastatic breast tumor cell behavior. The results of reverse transcriptase-polymerase chain reaction, Southern blot, nucleotide sequencing, immunoprecipitation, and immunoblot analyses indicated that these cells express a major CD44 isoform (molecular weight É 260 kDa) containing a v3,8-10 exon insertion (designated as CD44v 3,8-10 ). In addition, we have determined that CD44v 3,8-10 binds specifically to the cytoskeletal proteins such as ankyrin. Biochemical analyses, using competition binding assays and a synthetic peptide identical to NGGNGTVEDRKPSEL (a sequence located between aa480 and aa494 of CD44v 3,8-10 ) indicate that this 15-amino acid peptide binds specifically to the cytoskeletal protein ankyrin (but not to fodrin or spectrin). This peptide competes effectively for ankyrin binding to CD44v 3,8-10 . Therefore, we believe that the sequence 480 NGGNGTVEDRKPSE 494 L, located at the cytoplasmic domain of CD44v 3,

Author

Lilly Y. W. Bourguignon; Zeenat Gunja-Smith; Naoko Iida; H. B. Zhu; L. J. T. Young; William J. Muller; R. D. Cardiff

Publisher

John Wiley and Sons; Wiley (John Wiley & Sons); John Wiley & Sons Inc.; Wiley (ISSN 0021-9541)

Published

1998

Language

EN

Field

Biochemistry, Genetics and Molecular Biology (Life Sciences)