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Molecular systematics of the parasitic protozoan Giardia intestinalis by P. T. Monis; R. H. Andrews; G. Mayrhofer; P. L. Ey is a Biochemistry, Genetics and Molecular Biology article available to read on EtoBox.

What is Molecular systematics of the parasitic protozoan Giardia intestinalis about?

The long-standing controversy regarding whether Giardia intestinalis is a single species prevalent in both human and animal hosts or a species complex consisting of morphologically similar organisms that differ in host range and other biotypic characteristics is an issue with important medical, veterinary, and environmental management implications. In the past decade, highly distinct genotypes (some apparently confined to particular host groups) have been identified by genetic analysis of samples isolated from different host species. The aim of this study was to undertake a phylogenetic analysis of G. intestinalis that were representative of all known major genetic groups and compare them with other Giardia species, viz. G. ardeae, G. muris, and G. microti. Segments from four ''housekeeping'' genes (specifying glutamate dehydrogenase, triose phosphate isomerase, elongation factor 1␣, and 18S ribosomal RNA) were examined by analysis of 0.48-0.69-kb nucleotide sequences determined from DNA amplified in polymerase chain reactions from each locus. In addition, isolates were compared by allozymic analysis of electrophoretic data obtained for 21 enzymes representing 23 gene loci. The res

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Author

P. T. Monis; R. H. Andrews; G. Mayrhofer; P. L. Ey

Publisher

Oxford University Press (OUP)

Published

1999

Language

EN

Field

Biochemistry, Genetics and Molecular Biology (Life Sciences)