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Hepatitis C virus expression and interferon antiviral action is dependent on PKR expression by Yoshio Tokumoto; Yoichi Hiasa; Norio Horiike; Kojiro Michitaka; Bunzo Matsuura; Raymond T. Chung; Morikazu Onji is a Medicine article available to read on EtoBox.

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## Abstract Interferon (IFN)‐inducible double‐stranded RNA‐activated protein kinase (PKR) is thought to play a key antiviral role against hepatitis C virus (HCV). However, demonstrating the importance of PKR expression on HCV protein synthesis in the presence or absence of IFN has proven difficult in vivo. In the present experiment, full‐length HCV constructs were transiently transfected into two cell lines stably expressing T7 RNA polymerase. HCV expression was monitored under conditions of upregulated or downregulated PKR expression. In addition, IFN was monitored during downregulation of PKR. HCV expression effectively increased PKR expression, as well as that of its regulated proteins. PKR was obviously knocked down by PKR‐specific siRNA, which resulted in significantly increased HCV core protein levels. Conversely, over‐expression of PKR significantly suppressed HCV core levels in both cell lines. Furthermore, IFN induced high levels of PKR, whereas downregulation of PKR reversed IFN's antiviral effects and increased HCV core levels. Based on these results, it appears that HCV protein expression is directly dependent on PKR expression. PKR is antiviral toward HCV and responsib

Who reads Hepatitis C virus expression and interferon antiviral action is dependent on PKR expression?

Author

Yoshio Tokumoto; Yoichi Hiasa; Norio Horiike; Kojiro Michitaka; Bunzo Matsuura; Raymond T. Chung; Morikazu Onji

Publisher

John Wiley and Sons; Wiley (John Wiley & Sons); John Wiley & Sons Inc.; Wiley; Research Square (ISSN 0146-6615)

Published

2007

Language

EN

Field

Medicine (Health Sciences)