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A new structural type for Haemophilus influenzae lipopolysaccharide: **Structural analysis of the lipopolysaccharide from nontypeable Haemophilus influenzae strain 486** by Martin Månsson; Sebastian H. J. Bauer; Derek W. Hood; James C. Richards; E. Richard Moxon; Elke K. H. Schweda is a Biochemistry, Genetics and Molecular Biology article available to read on EtoBox.

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Structural elucidation of the sialylated lipopolysaccharide (LPS) of non‐typeable __Haemophilus influenzae__ (NTHi) strain 486 has been achieved by the application of high‐field NMR techniques and ESI‐MS along with composition and linkage analyses on O‐deacylated LPS and oligosaccharide samples. It was found that the LPS contains the common element of __H. influenzae__, l‐α‐d‐Hep__p__‐(1→2)‐[__P__Etn→6]‐l‐α‐d‐Hep__p__‐(1→3)‐[β‐d‐Glc__p__‐(1→4)]‐l‐α‐d‐Hep__p__‐(1→5)‐[__PP__Etn→4]‐α‐Kdo__p__‐(2→6)‐Lipid A, but instead of glycosyl substitution of the terminal heptose residue (HepIII) at the O2 position observed in other __H. influenzae__ strains, HepIII is chain elongated at the O3 position by either lactose or sialyllactose (i.e. α‐Neu5Ac‐(2→3)‐β‐d‐Gal__p__‐(1→4)‐β‐d‐Glc__p__). The LPS is substituted by an O‐acetyl group linked to the O2 position of HepIII and phosphocholine (__P__Cho) which was located at the O6 position of a terminal α‐d‐Glc__p__ residue attached to the central heptose, a molecular environment different from what has been reported earlier for __P__Cho. In addition, minor substitution by O‐linked glycine to the LPS was observed. By investigation of LPS from a __lpsA

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Author

Martin Månsson; Sebastian H. J. Bauer; Derek W. Hood; James C. Richards; E. Richard Moxon; Elke K. H. Schweda

Publisher

John Wiley and Sons; Springer; Wiley (Blackwell Publishing); Springer-Verlag; Blackwell Publishing Inc.; Springer Verlag; Wiley; Springer Science and Business Media LLC (ISSN 1432-1327)

Published

2001

Language

EN

Field

Biochemistry, Genetics and Molecular Biology (Life Sciences)