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Can I read Immobilized Triton X-100-assisted refolding of Green Fluorescent Protein-Tobacco Etch Virus protease fusion protein using β-cyclodextrin as the eluent on EtoBox?
Immobilized Triton X-100-assisted refolding of Green Fluorescent Protein-Tobacco Etch Virus protease fusion protein using β-cyclodextrin as the eluent by Jing-Jing Li; Ai-Qing Wang; Jan-Christer Janson; Andras Ballagi; Jing Chen; Yong-Dong Liu; Guang-Hui Ma; Zhi-Guo Su is a Biochemistry, Genetics and Molecular Biology article available to read on EtoBox.
What is Immobilized Triton X-100-assisted refolding of Green Fluorescent Protein-Tobacco Etch Virus protease fusion protein using β-cyclodextrin as the eluent about?
A new protein refolding technique based on the use of the non-charged detergent Triton X-100 immobilized to the cross-linked agarose gel Sepharose High Performance has been developed. The new solid phase was used in combination with soluble b-cyclodextrin (b-CD) to refold recombinant Green Fluorescent Protein fused to Tobacco Etch Virus protease (GFPTEVP) expressed as inclusion bodies in E. coli. Previous attempts to refold recombinant GFPTEVP by dilution had failed. In the new procedure a column packed with Triton X-100-coupled Sepharose High Performance was used to capture unfolded GFPTEVP followed by elution using an increasing b-CD concentration gradient. The yield of properly refolded GFPTEVP was 46% at a protein concentration of 380 mg/ml. In contrast, dilution refolding of GFPTEVP at 200 mg/ml refolding buffer resulted in only 4.7% of native protein.
Who reads Immobilized Triton X-100-assisted refolding of Green Fluorescent Protein-Tobacco Etch Virus protease fusion protein using β-cyclodextrin as the eluent?
It is typically read by researchers, students, and practitioners in Biochemistry, Genetics and Molecular Biology.
- Author
- Jing-Jing Li; Ai-Qing Wang; Jan-Christer Janson; Andras Ballagi; Jing Chen; Yong-Dong Liu; Guang-Hui Ma; Zhi-Guo Su
- Publisher
- Elsevier Science; Elsevier ; Elsevier Applied Science; Elsevier BV (ISSN 1873-3298)
- Published
- 2009
- Language
- EN
- Field
- Biochemistry, Genetics and Molecular Biology (Life Sciences)