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Macrophages Induce Cardiomyocyte Ferroptosis via Mitochondrial Transfer by Jun Chen,Chun-yan Fu,Gerong Shen,Jingyu Wang,Lintao Xu,Heyangzi Li,Xi Cao,Ming-zhi Zheng,Yue-liang Shen,Jinjie Zhong,Ying-ying Chen,Lin-lin Wang is a nonfiction available to read on EtoBox.

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Free Radical Biology and Medicine, 190 (2022) 1-14. doi:10.1016/j.freeradbiomed.2022.07.015 Macrophages induce cardiomyocyte ferroptosis via mitochondrial transfer 1 1 Introduction 1 2 Materials and methods 2 2.1 Animals and cell lines 2 2.2 Reagents 2 2.3 Animal grouping 2 2.4 Immunofluorescence staining of cardiac tissue 2 2.5 Primary culture of neonatal mouse cardiomyocytes 2 2.6 Preparation of macrophage culture medium 2 2.7 Isolation and culture of bone marrow-derived macrophages 2 2.8 Cardiomyocyte treatment 2 2.9 Detection of macrophage-originated mitochondria in cardiomyocytes 3 2.10 Quantitative analysis of cardiomyocytes internalized macrophage-derived mitochondria 3 2.11 Observation of formed elements in macrophage-conditioned medium 3 2.12 Analysis of mitochondrial membrane potential 3 2.13 Evaluation of apoptosis 3 2.14 Observation of mitochondrial morphology 3 2.15 Assessment of ferrous ion concentration and lipid peroxidation content 3 2.16 ATP assay 3 2.17 GSH/GSSG ratio assay 3 2.18 Extraction of total RNA 3 2.19 Quantitative real-time PCR assay 3 2.20 RNA sequencing and differentially expressed mRNAs analysis 5 2.21 GO, KEGG, and GSEA analysis 5 2.22 Untargeted me

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Author

Jun Chen,Chun-yan Fu,Gerong Shen,Jingyu Wang,Lintao Xu,Heyangzi Li,Xi Cao,Ming-zhi Zheng,Yue-liang Shen,Jinjie Zhong,Ying-ying Chen,Lin-lin Wang

Publisher

Elsevier Inc.

Language

EN

Category

nonfiction