Can I read Purification of a Cholecystokinin Fragment Peptide in the Horizontal Flow-through Coil Planet Centrifuge on EtoBox?

Purification of a Cholecystokinin Fragment Peptide in the Horizontal Flow-through Coil Planet Centrifuge by Martha Knight; Carol A. Tamminga; Yoichiro Ito; Jerry D. Gardner; Thomas N. Chase is a Chemistry article available to read on EtoBox.

What is Purification of a Cholecystokinin Fragment Peptide in the Horizontal Flow-through Coil Planet Centrifuge about?

For the preparation of cholecystokinin (CCK) fragment peptides and their analogs which have been found to be inhibitors of its activity's2 we have been using a novel method of counter-current chromatography (CCC) carried out in the horizontal flow-through coil planet centrifuge for purifying the synthetic peptides. Described here are the modifications of CCC solvent systems for the fractionation of Ac-Tyr-Met-Gly-Trp-Met-Tfa Lys and the sulfated and deprotected product, Ac-Tyr-(S03H)-Met-Gly-Trp-Lys ([Lys3 ']Ac-CCK 2631). The horizontal flow-through coil planet centrifuge is an instrument designed for equilibration of a two-phase solvent system in a centrifugal force field3. One phase is retained while the other mobile phase is eluted and substances are separated according to their partition coefficients. This method has been previously described for the purification of endorphin peptides4. Ac-Asp-Tyr-Met-Gly-Trp-Tfa Lys was synthesized by solid-phase methods. The peptide was purified by CCC and subsequently sulfated5. The incorporation of sulfated tyrosine directly during the solid-phase synthesis would have required the avoidance of acid reagents for the deprotection and peptide-

Who reads Purification of a Cholecystokinin Fragment Peptide in the Horizontal Flow-through Coil Planet Centrifuge?

Author

Martha Knight; Carol A. Tamminga; Yoichiro Ito; Jerry D. Gardner; Thomas N. Chase

Publisher

Elsevier Science; Elsevier ; Elsevier BV (ISSN 1873-3778)

Published

1984

Language

EN

Field

Chemistry (Physical Sciences)