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Can I read Quantitative Multiple Fragment Monitoring with Enhanced In-source Fragmentation/annotation Mass Spectrometry on EtoBox?
Quantitative Multiple Fragment Monitoring with Enhanced In-source Fragmentation/annotation Mass Spectrometry by Samuel Bernardo-Bermejo; Jingchuan Xue; Linh Hoang; Elizabeth Billings; Bill Webb; M. Willy Honders; Sanne Venneker; Bram Heijs; María Castro-Puyana; María Luisa Marina; Erik B. van den Akker; Marieke Griffioen; Gary Siuzdak; Martin Giera; Elena Sánchez-López is a Biochemistry, Genetics and Molecular Biology article available to read on EtoBox.
What is Quantitative Multiple Fragment Monitoring with Enhanced In-source Fragmentation/annotation Mass Spectrometry about?
Analytical techniques with high sensitivity and selectivity are essential to the quantitative analysis of clinical samples. Liquid chromatography coupled to tandem mass spectrometry is the gold standard in clinical chemistry. However, tandem mass spectrometers come at high capital expenditure and maintenance costs. We recently showed that it is possible to generate very similar results using a much simpler single mass spectrometry detector by performing enhanced in-source fragmentation/annotation (EISA) combined with correlated ion monitoring. Here we provide a step-by-step protocol for optimizing the analytical conditions for EISA, so anyone properly trained in liquid chromatography-mass spectrometry can follow and apply this technique for any given analyte. We exemplify the approach by using 2-hydroxyglutarate (2-HG) which is a clinically relevant metabolite whose D-enantiomer is considered an 'oncometabolite', characteristic of cancers associated with mutated isocitrate dehydrogenases 1 or 2 (IDH1/2). We include procedures for determining quantitative robustness, and show results of these relating to the analysis of DL-2-hydroxyglutarate in cells, as well as in serum samples fro
Who reads Quantitative Multiple Fragment Monitoring with Enhanced In-source Fragmentation/annotation Mass Spectrometry?
It is typically read by researchers, students, and practitioners in Biochemistry, Genetics and Molecular Biology.
- Author
- Samuel Bernardo-Bermejo; Jingchuan Xue; Linh Hoang; Elizabeth Billings; Bill Webb; M. Willy Honders; Sanne Venneker; Bram Heijs; María Castro-Puyana; María Luisa Marina; Erik B. van den Akker; Marieke Griffioen; Gary Siuzdak; Martin Giera; Elena Sánchez-López
- Publisher
- Springer Science and Business Media LLC
- Published
- 2023
- Language
- EN
- Field
- Biochemistry, Genetics and Molecular Biology (Life Sciences)