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Generation of Osteoclasts from Hemopoietic Cells and a Multipotential Cell Line in Vitro by G. Hattersley; T. J. Chambers is a Biochemistry, Genetics and Molecular Biology article available to read on EtoBox.

What is Generation of Osteoclasts from Hemopoietic Cells and a Multipotential Cell Line in Vitro about?

Osteoclasts are the cells that resorb bone. It is generally presumed, on the basis of indirect experiments, that they are derived from the hemopoietic stem cell. However, this origin has never been established. We have developed an assay for osteoclastic differentiation in which bone marrow cells are incubated in liquid culture on slices of cortical bone. The bone slices are inspected in the scanning electron microscope after incubation for the presence of excavations, which are characteristic of osteoclastic activity. We have now incubated bone marrow cells at low density, or a factor-dependent mouse hemopoietic cell line (FDCP-mix A4) with 1,25 dihydroxyvitamin D3 (a hormone which we have previously found induces osteoclastic differentiation) with and without murine bone marrow stro-ma1 cells, or with and without 3T3 cells, on bone slices. Neither the bone marrow cells nor the bone marrow stromal cells alone developed osteoclastic function even in the presence of 1,25 dihydroxyvitamin D3. However, extensive excavation of the bone surface was observed, only in the presence of 1,25 dihydroxyvitamin D3, on bone slices on which bone marrow stromal cells were cocultured with low-densi

Who reads Generation of Osteoclasts from Hemopoietic Cells and a Multipotential Cell Line in Vitro?

Author

G. Hattersley; T. J. Chambers

Publisher

John Wiley and Sons; Wiley (John Wiley & Sons); John Wiley & Sons Inc.; Wiley (ISSN 0021-9541)

Published

1989

Language

EN

Field

Biochemistry, Genetics and Molecular Biology (Life Sciences)