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RNA-seq: the premier transcriptomics tool by Robert E. Farrell Jr is a book available to read on EtoBox.

What is RNA-seq: the premier transcriptomics tool about?

RNA-seq is an amalgam of standard molecular biology techniques and computational bioinformatics that are designed to identify and quantify transcriptional activity in the cell. The nomenclature, RNA-seq, comes from "RNA sequence analysis" and the technology is a widely regarded asset within the scientific community. The utility and popularity of this transcriptomics tool are based on the high-throughput and massively parallel character of NGS (next-generation sequencing) and the unique characteristics of third-generation sequencing which are exemplified by RNA-seq. RNA-seq has revolutionized and invigorated the mining of the transcriptome for its secrets by providing much greater latitude in experimental design and providing an understanding of the transcriptome at heretofore unknown levels of detail. This represents an unprecedented opportunity to further refine the current models of gene expression. In addition to the coding, noncoding, and intergenic RNAs that are already known, it is likely that there is a treasure trove of unannotated transcripts awaiting characterization.RNA-seq produces a quantitative and a qualitative profile of each sample. The quantitative profile reflect

Author

Robert E. Farrell Jr

Publisher

Elsevier Science & Technology; Academic Press

Published

2023

Language

EN

ISBN

9780323916561

Subjects

Science, Medical, Biology