Can I read Fob1-dependent condensin recruitment and loop extrusion on yeast chromosome III on EtoBox?

Fob1-dependent condensin recruitment and loop extrusion on yeast chromosome III by Manikarna Dinda; Ryan D. Fine; Shekhar Saha; Zhenjia Wang; Chongzhi Zang; Mingguang Li; Jeffrey S. Smith is a Biochemistry, Genetics and Molecular Biology article available to read on EtoBox.

What is Fob1-dependent condensin recruitment and loop extrusion on yeast chromosome III about?

Despite recent advances in single-molecule and structural analysis of condensin activity __in vitro__, mechanisms of functional condensin loading and loop extrusion that lead to specific chromosomal organization remain unclear. In __Saccharomyces cerevisiae__, the most prominent condensin loading site is the rDNA locus on chromosome XII, but its repetitiveness deters rigorous analysis of individual genes. An equally prominent non-rDNA condensin site is located on chromosome III (chrIII). It lies in the promoter of a putative non-coding RNA gene called __RDT1__, which is in a segment of the recombination enhancer (RE) that dictates __MAT__a-specific chrIII organization. Here, we unexpectedly find that condensin is recruited to the __RDT1__ promoter in __MAT__a cells through hierarchical interactions with Fob1, Tof2, and cohibin (Lrs4/Csm1), a set of nucleolar factors that also recruit condensin to the rDNA. Fob1 directly binds to this locus __in vitro__, while its binding __in vivo__ depends on an adjacent Mcm1/α2 binding site that provides __MAT__a cell specificity. We also uncover evidence for condensin-driven loop extrusion anchored by Fob1 and cohibin at __RDT1__ that unidirecti

Who reads Fob1-dependent condensin recruitment and loop extrusion on yeast chromosome III?

Author

Manikarna Dinda; Ryan D. Fine; Shekhar Saha; Zhenjia Wang; Chongzhi Zang; Mingguang Li; Jeffrey S. Smith

Publisher

Public Library of Science (PLoS)

Published

2023

Language

EN

Field

Biochemistry, Genetics and Molecular Biology (Life Sciences)