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Alkaline Phosphatase of Basal Lateral and Brush Border Plasma Membranes from Intestinal Epithelium by S. D. Hanna; A. K. Mircheff; E. M. Wright is a Biochemistry, Genetics and Molecular Biology article available to read on EtoBox.

What is Alkaline Phosphatase of Basal Lateral and Brush Border Plasma Membranes from Intestinal Epithelium about?

The alkaline phosphatases present on isolated brush border and basal lateral membranes of rat duodenal epitheilum were examined by means of a variety of biochemical assays and physical methods. The two alkaline phosphatases have similar pH optima of 9.6-9.8, similar substrate km's for p-nitrophenyl phosphate (PNPP) of 7 1 micromolar, similar responses t o the inhibitors 2-mercaptoethanol, theophylline, phenylalanine, and ethylenediaminetetraacetic acid (EDTA), similar sensitivities t o calcium, magnesium, zinc, sodium, and potassium, and similar insensitivities t o digestion with trypsin or papain. The two enzymes also exhibit similar molecular weights on SDS-polyacrylamide gels in the range 124,000-150,000, and both enzymes show an Rf value of 0.092 on Triton X-1 00 polyacrylamide gels, indicating similar intrinsic charges. The Vmax of the brush border enzyme is ten times greater than that of the basal lateral enzyme, 140 pmoles/mg-h as opposed to 14 pmoles/mg-h. The differences in Vma, are a reflection of the known distribution of alkaline phosphatase in rat duodenum, there being more alkaline phosphatase activity present on the brush border than on the basal lateral surface. One

Who reads Alkaline Phosphatase of Basal Lateral and Brush Border Plasma Membranes from Intestinal Epithelium?

Author

S. D. Hanna; A. K. Mircheff; E. M. Wright

Publisher

Wiley (John Wiley & Sons); Alan R. Liss, Inc.; Wiley (ISSN 0091-7419)

Published

1978

Language

EN

Field

Biochemistry, Genetics and Molecular Biology (Life Sciences)