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Characterization of Spatial Growth and Distribution of Chondrocyte Cells Embedded in Collagen Gels Through a Stereoscopic Cell Imaging System by Masahiro Kino-oka; Yoshikatsu Maeda; Yasuaki Sato; Ali Baradar Khoshfetrat; Takeyuki Yamamoto; Katsura Sugawara; Masahito Taya is a Biochemistry, Genetics and Molecular Biology article available to read on EtoBox.
What is Characterization of Spatial Growth and Distribution of Chondrocyte Cells Embedded in Collagen Gels Through a Stereoscopic Cell Imaging System about?
## Abstract The stereoscopic image analysis of fluorescence‐labeled chondrocyte cells for cytoplasm and nucleus was performed for the quantitative determination of spatial cell distribution as well as cell aggregate size in the collagen‐embedded culture. The three‐dimensional histomorphometric data indicated that the cells in the gels formed aggregates by cell division, and the size of aggregates increased with elapsed culture time. In the culture seeded at 2.0 × 10^6^ cells/cm^3^, the cells showed a semilunar shape that is a typical chondrocytic morphology, and formed the dense cell aggregates producing collagen type II. From the quantitative analysis of aggregate size, in addition, it was found that the cell division caused the aggregate growth with an increase of cell number in respective aggregates at 7 days, and some of aggregates made coalescence at 14 days. In the gel surface region, further coalescence of aggregates accompanied with cell division produced larger cell clusters, creating cell layers on the gel surface at the end of culture (21 days). In the culture seeded at 2.0 × 10^5^ cells/cm^3^, the different manner of aggregation was observed. At 14 days, the loose clust
Who reads Characterization of Spatial Growth and Distribution of Chondrocyte Cells Embedded in Collagen Gels Through a Stereoscopic Cell Imaging System?
It is typically read by researchers, students, and practitioners in Biochemistry, Genetics and Molecular Biology.
- Author
- Masahiro Kino-oka; Yoshikatsu Maeda; Yasuaki Sato; Ali Baradar Khoshfetrat; Takeyuki Yamamoto; Katsura Sugawara; Masahito Taya
- Publisher
- John Wiley and Sons; Wiley (John Wiley & Sons); Wiley - V C H Verlag GmbbH & Co.; Wiley (ISSN 0006-3592)
- Published
- 2007
- Language
- EN
- Field
- Biochemistry, Genetics and Molecular Biology (Life Sciences)