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DNA barcode and identification of the varieties and provenances of Taiwan’s domestic and imported made teas using ribosomal internal transcribed spacer 2 sequences by Shih-Chieh Lee; Chia-Hsiang Wang; Cheng-En Yen; Chieh Chang is a Medicine article available to read on EtoBox.

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The major aim of made tea identification is to identify the variety and provenance of the tea plant. The present experiment used 113 tea plants [__Camellia sinensis__ (L.) O. Kuntze] housed at the Tea Research and Extension Substation, from which 113 internal transcribed spacer 2 (ITS2) fragments, 104 __trnL__ intron, and 98 __trnL-trnF__ intergenic sequence region DNA sequences were successfully sequenced. The similarity of the ITS2 nucleotide sequences between tea plants housed at the Tea Research and Extension Substation was 0.379–0.994. In this polymerase chain reaction-amplified noncoding region, no varieties possessed identical sequences. Compared with the __trnL__ intron and __trnL-trnF__ intergenic sequence fragments of chloroplast cpDNA, the proportion of ITS2 nucleotide sequence variation was large and is more suitable for establishing a DNA barcode database to identify tea plant varieties. After establishing the database, 30 imported teas and 35 domestic made teas were used in this model system to explore the feasibility of using ITS2 sequences to identify the varieties and provenances of made teas. A phylogenetic tree was constructed using ITS2 sequences with the unweig

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Author

Shih-Chieh Lee; Chia-Hsiang Wang; Cheng-En Yen; Chieh Chang

Publisher

The Journal of Food and Drug Analysis (JFDA), Food and Drug Administration, Taiwan (TFDA)

Published

2017

Language

EN

Field

Medicine (Life Sciences)