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Can I read High-Throughput Production of Diverse Xenobiotic Metabolites with Cytochrome P450–Transduced Huh7 Hepatoma Cell Lines on EtoBox?
High-Throughput Production of Diverse Xenobiotic Metabolites with Cytochrome P450–Transduced Huh7 Hepatoma Cell Lines by Choon-myung Lee; Ken H. Liu; Grant Singer; Gary W. Miller; Shuzhao Li; Dean P. Jones; Edward T. Morgan is a Medicine article available to read on EtoBox.
What is High-Throughput Production of Diverse Xenobiotic Metabolites with Cytochrome P450–Transduced Huh7 Hepatoma Cell Lines about?
Precision medicine and exposomics require methods to assess xenobiotic metabolism in human metabolomic analyses, including the identification of known and undocumented drug and chemical exposures as well as their metabolites. Recent work demonstrated the use of high-throughput generation of xenobiotic metabolites with human liver S-9 fractions for their detection in human plasma and urine. Here, we tested whether a panel of lentivirally transduced human hepatoma cell lines (Huh7) that express individual cytochrome P450 (P450) enzymes could be used to generate P450-specific metabolites in a high-throughput manner, while simultaneously identifying the enzymes responsible. Cell-line activities were verified using P450-specific probe substrates. To increase analytical throughput, we used a pooling strategy where 36 chemicals were grouped into 12 unique mixtures, each mixture containing 6 randomly selected compounds, and each compound being present in two separate mixtures. Each mixture was incubated with 8 different P450 cell lines for 0 and 2 hours and extracts were analyzed using liquid chromatography-high-resolution mass spectrometry. Cell lines selectively metabolized test substrat
Who reads High-Throughput Production of Diverse Xenobiotic Metabolites with Cytochrome P450–Transduced Huh7 Hepatoma Cell Lines?
It is typically read by researchers, students, and practitioners in Medicine.
- Author
- Choon-myung Lee; Ken H. Liu; Grant Singer; Gary W. Miller; Shuzhao Li; Dean P. Jones; Edward T. Morgan
- Publisher
- American Society for Pharmacology & Experimental Therapeutics (ASPET)
- Published
- 2022
- Language
- EN
- Field
- Medicine (Life Sciences)