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A HU‐like gene mutation in Rhizobium leguminosarum bv. viciae affects the expression of nodulation genes by Qiang Li; Jie Feng; Hai‐Liang Hu; Xiao‐Chun Chen; Feng‐Qing Li; Guo‐Fan Hong is a Biochemistry, Genetics and Molecular Biology article available to read on EtoBox.

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## Summary NodD is the major regulator of __nod__ genes expression in rhizobia. Previously, a HU‐like protein in __Rhizobium leguminosarum__ bv. __viciae__ has been identified to bind specifically with __nod__ promoters and be involved in __in vitro nodD__ transcription, but its __in vivo__ function remained unknown. In this work we have cloned and sequenced the __R__. __leguminosarum__ bv. __viciae__ gene, named __hurL__, for this HU‐like protein. Using the __E__. __coli__‐expressed HurL proteins, we proved that HurL had high affinity to several __nod__ promoters and showed a stimulation effect on __in vitro nodD__ transcription at appropriate concentration. The __R__. __leguminosarum__ bv. __viciae hurL__ gene was mutated by insertion of a kanamycin resistance cassette. The obtained __hurL__ mutant strain M704 exhibited poor growth under free‐living conditions and failed to induce nodules on __Pisum sativum__ cv. __Frisson__ and __Vicia hirsuta__. Further studies of NodD production and __nod__ genes‐__lacZ__ fusions expression in the __hurL__ mutant revealed that inactivation of __hurL__ led to severe impairment in the __nodD__ expression, repression in the inducible expression o

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Author

Qiang Li; Jie Feng; Hai‐Liang Hu; Xiao‐Chun Chen; Feng‐Qing Li; Guo‐Fan Hong

Publisher

John Wiley and Sons; Wiley (Blackwell Publishing); Blackwell Publishing Inc.; Wiley (ISSN 0950-382X)

Published

2003

Field

Biochemistry, Genetics and Molecular Biology (Life Sciences)