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Zinc Transport Into Endothelial Cells Is a Facilitated Process by Dennis J. Bobilya; Mary Briske-Anderson; Philip G. Reeves is a Biochemistry, Genetics and Molecular Biology article available to read on EtoBox.

What is Zinc Transport Into Endothelial Cells Is a Facilitated Process about?

The kinetics of zinc transport were examined by measuring the uptake of ""Zn into cultured endothelial cells. This served as a suitable model for characterizing the transport of zinc across a biological membrane (i.e., t h e plasma membrane). The transport process was saturable under physiological conditions, which indicates a facilitating transport mechanism. Within the physiological range of zinc concentrations, the maximum zinc transport rate was 27 pmoles zinc/(min x mg protein) and it was half maximal at 4.1 pM zinc. Cadmium competitively inhibited zinc transport (Ki = 6.5 pM), while equimolar concentrations of copper and manganese were ineffectual. The rate of zinc transport was substantially reduced at lower temperatures and in the presence of sulfhydryl blockers (sodium iodoacetate and N-ethylmaleimide). Inhibitors of energy metabolism (2,4-dinitrophenol and sodium azide) failed to disrupt zinc transport. These results demonstrate that zinc transport into endothelial cells is a facilitated process (i.e., it is carrier mediated and energy-independent). o I 992 WiIey-Liss, Inc.

Who reads Zinc Transport Into Endothelial Cells Is a Facilitated Process?

Author

Dennis J. Bobilya; Mary Briske-Anderson; Philip G. Reeves

Publisher

John Wiley and Sons; Wiley (John Wiley & Sons); John Wiley & Sons Inc.; Wiley (ISSN 0021-9541)

Published

1992

Language

EN

Field

Biochemistry, Genetics and Molecular Biology (Life Sciences)