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Developing a Non-destructive Metabarcoding Protocol for Detection of Pest Insects in Bulk Trap Catches by Batovska, Jana (author);Piper, Alexander M. (author);Valenzuela, Isabel (author);Cunningham, John Paul (author);Blacket, Mark J. (author) is a Biochemistry, Genetics and Molecular Biology article available to read on EtoBox.
What is Developing a Non-destructive Metabarcoding Protocol for Detection of Pest Insects in Bulk Trap Catches about?
Metabarcoding has the potential to revolutionise insect surveillance by providing high-throughput and cost-effective species identification of all specimens within mixed trap catches. Nevertheless, incorporation of metabarcoding into insect diagnostic laboratories will first require the development and evaluation of protocols that adhere to the specialised regulatory requirements of invasive species surveillance. In this study, we develop a multi-locus non-destructive metabarcoding protocol that allows sensitive detection of agricultural pests, and subsequent confirmation using traditional diagnostic techniques. We validate this protocol for the detection of tomato potato psyllid (__Bactericera cockerelli__) and Russian wheat aphid (__Diuraphis noxia__) within mock communities and field survey traps. We find that metabarcoding can reliably detect target insects within mixed community samples, including specimens that morphological identification did not initially detect, but sensitivity appears inversely related to community size and is impacted by primer biases, target loci, and sample indexing strategy. While our multi-locus approach allowed independent validation of target detec
Who reads Developing a Non-destructive Metabarcoding Protocol for Detection of Pest Insects in Bulk Trap Catches?
It is typically read by researchers, students, and practitioners in Biochemistry, Genetics and Molecular Biology.
- Author
- Batovska, Jana (author);Piper, Alexander M. (author);Valenzuela, Isabel (author);Cunningham, John Paul (author);Blacket, Mark J. (author)
- Publisher
- Springer Science and Business Media LLC
- Published
- 2021
- Language
- EN
- Field
- Biochemistry, Genetics and Molecular Biology (Life Sciences)