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Detection of cytolethal distending toxin activity and cdt genes in Actinobacillus actinomycetemcomitans isolates from geographically diverse populations by A. S. Fabris; J. M. DiRienzo; M. Wïkstrom; M. P. A. Mayer is a Dentistry article available to read on EtoBox.

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A cytolethal distending toxin (CDT) found in __Actinobacillus actinomycetemcomitans__ inhibits the eukaryotic cell cycle, which may contribute to the pathogenic potential of the bacterium. The presence of the __cdtABC__ genes and CDT activity were examined in 40 clinical isolates of __A. actinomycetemcomitans__ from Brazil, Kenya, Japan and Sweden. Thirty‐nine of 40 cell lysates caused distension of Chinese hamster ovary cells. At least one of the __cdt__ genes was detected in all strains examined. The three __cdt__ genes were detected, by PCR, in 34 DNA samples. DNA from one strain from Kenya did not yield amplicons of the __cdtA__ and __cdtB__ genes and did not express toxic activity. Restriction analysis was performed on every amplicon obtained. PCR‐RFLP patterns revealed that the three __cdt__ genes were conserved. These data provided evidence that the __cdt__ genes are found and expressed in the majority of the __A. actinomycetemcomitans__ isolates. Although a quantitative difference in cytotoxicity was observed, indicating variation in expression of CDT among strains, no clear relationship between CDT activity and periodontal status was found.

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Author

A. S. Fabris; J. M. DiRienzo; M. Wïkstrom; M. P. A. Mayer

Publisher

John Wiley and Sons; Wiley (Blackwell Publishing); Blackwell Publishing Inc.; Wiley (ISSN 0902-0055)

Published

2002

Language

EN

Field

Dentistry (Health Sciences)