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Synthetic (glyco-)peptides of the homophilic recognition domain of E-cadherin lead to increased E-cadherin mRNA synthesis and are inductors of cell differentiation in primary lung cancer cell lines by Christian Zeckey; Manfred Dahm; Anja Wallrath; Monika Herr; Horst Walter Kunz; Christian-Friedrich Vahl; Sigrid Horn is a Medicine article available to read on EtoBox.
What is Synthetic (glyco-)peptides of the homophilic recognition domain of E-cadherin lead to increased E-cadherin mRNA synthesis and are inductors of cell differentiation in primary lung cancer cell lines about?
E-cadherin is one of the critical molecules involved in the metastatic process in many types of cancer. Once combined, E-cadherin exceeds the amount of membranous E-cadherin on the cellular surface by activation of intracellular signaling cascades. Studies on transformed keratinocytes of the HaCat cell line showed induction of differentiation by synthetical partial structures of the homophilic binding region of E-cadherin. The knowledge of effects in lung cancer cells is sparse. Therefore, the effects in primary lung cancer cell lines were investigated. Four primary lung cancer cell lines were incubated for 3, 6, 12, 15, 18, and 24 h with synthetic partial structures (peptide and glycopeptide). The control substance was sodium butyrate. mRNA was isolated, and relative quantification of E-cadherin was performed using the Real-Time PCR. During the stimulation period, morphologic pictures were taken, and immunohistochemical staining of membranous E-cadherin was performed. Life/dead assays were used to display cell vitality. The intracellular E-cadherin mRNA amount was increased after incubation with the synthetic partial structures. Life/dead assays showed improved survival and integr
Who reads Synthetic (glyco-)peptides of the homophilic recognition domain of E-cadherin lead to increased E-cadherin mRNA synthesis and are inductors of cell differentiation in primary lung cancer cell lines?
It is typically read by researchers, students, and practitioners in Medicine.
- Author
- Christian Zeckey; Manfred Dahm; Anja Wallrath; Monika Herr; Horst Walter Kunz; Christian-Friedrich Vahl; Sigrid Horn
- Publisher
- Elsevier Science; Elsevier ; Elsevier BV (ISSN 0344-0338)
- Published
- 2010
- Language
- EN
- Field
- Medicine (Health Sciences)