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Imaging Mass Spectrometry to Visualise Increased Acetylcholine in Lungs of Asthma Model Mice by Takeshi Matsuda; Yuzo Suzuki; Tomoyuki Fujisawa; Yasunori Suga; Nobuyuki Saito; Takafumi Suda; Ikuko Yao is a Chemistry article available to read on EtoBox.

What is Imaging Mass Spectrometry to Visualise Increased Acetylcholine in Lungs of Asthma Model Mice about?

Acetylcholine (ACh) is a crucial neurotransmitter that is involved in airway constriction. In fact, excessive ACh binding to M3 muscarinic receptor leads to airflow obstruction via smooth muscle contraction. Previous studies have suggested cholinergic malfunction in the pathogenesis of asthma; however, the distribution and abundance of ACh in asthmatic lungs remain unclear because of the challenges of imaging ACh in lung tissue. In this study, we successfully detected and visualised ACh in mouse lung tissue by using Fourier transform ion cyclotron resonance mass spectrometry (FT-ICR-MS). Here, we applied the ACh imaging method to the two groups of house dust mite-sensitised asthma model mice harbouring different inflammatory levels. The imaging results showed that the lungs of mice had a relatively uniform ACh distribution with some areas of heterogeneity. The lungs of asthma model mice had significantly more ACh than control mice, and the ACh increase was potentiated with intense eosinophil infiltration without acetylcholinesterase deficits. These results indicate that ACh hypersecretion is mediated by an increased infiltration of eosinophils in asthma aggravation. This study prov

Who reads Imaging Mass Spectrometry to Visualise Increased Acetylcholine in Lungs of Asthma Model Mice?

Author

Takeshi Matsuda; Yuzo Suzuki; Tomoyuki Fujisawa; Yasunori Suga; Nobuyuki Saito; Takafumi Suda; Ikuko Yao

Publisher

Springer; Springer-Verlag; Springer Verlag; Springer Science and Business Media LLC; Society for Mining, Metallurgy and Exploration Inc.; Springer Nature (ISSN 1618-2650)

Published

2020

Language

EN

Field

Chemistry (Physical Sciences)