Can I read Rapid Genotype Method to Distinguish Frequent and/or Functional Polymorphisms in Human N-Acetyltransferase-1 on EtoBox?

Rapid Genotype Method to Distinguish Frequent and/or Functional Polymorphisms in Human N-Acetyltransferase-1 by Mark A. Doll; David W. Hein is a Biochemistry, Genetics and Molecular Biology article available to read on EtoBox.

What is Rapid Genotype Method to Distinguish Frequent and/or Functional Polymorphisms in Human N-Acetyltransferase-1 about?

ppm), due to the ␤ effect. The same effect for compound 4 shifts the C-6 signal, which is observed at 66.2 ppm. Deprotection of HO-6 also affects the pattern of H-6,6Ј in the 1 H NMR spectrum of 3. In this case, the two protons appear as a doublet at the same chemical shift (4.07 ppm, Table 1). Oxidation of HO-6 was performed with PCC as described (12) affording 5 (88%). Compound 5 showed a singlet at 9.2 ppm in the 1 H NMR spectrum, and a signal at ␦ 196.5 in the 13 C NMR spectrum, both diag- nostic signals for the presence of the aldehyde group at C-6. For the reduction and debenzoylation steps, standard procedures were used. Compound [ 3 H]1 showed the same chromatographic properties by TLC (Fig. 1, lane I) and HPAEC-PAD (Fig. 2A) than the nonradioactive compound. The specific activity (13 Ci/mol) was calculated from the ratio between the radioactivity of the compound eluted from the HPAEC and the amount of material calculated by using mannose as standard. Compound [ 3 H]1 was used as substrate for the exo-␤-D-galactofuranosidase from P. fellutanum, under the usual conditions ( 6). The enzymatic reaction could be followed by TLC (Fig. 1, lane II) and HPAEC-PAD analysis (Fig. 2B)

Who reads Rapid Genotype Method to Distinguish Frequent and/or Functional Polymorphisms in Human N-Acetyltransferase-1?

Author

Mark A. Doll; David W. Hein

Publisher

Elsevier Science; Elsevier ; Elsevier Inc.; Elsevier BV (ISSN 0003-2697)

Published

2002

Language

EN

Field

Biochemistry, Genetics and Molecular Biology (Life Sciences)