Can I read Chromogranin A gene expression in non-small cell lung carcinomas on EtoBox?

Chromogranin A gene expression in non-small cell lung carcinomas by Giancarlo Abbona; Mauro Papotti; Laura Viberti; Luigia Macr??; Anna Stella; Gianni Bussolati is a Medicine article available to read on EtoBox.

What is Chromogranin A gene expression in non-small cell lung carcinomas about?

Evidence for the existence of neuroendocrine (NE) differentiation in non-small cell lung carcinomas (NSCLCs) is at present based on histochemical, ultrastructural, and immunohistochemical data. The aim of this study was to investigate the extent of NE differentiation in NSCLCs as revealed by mRNA analysis. Different techniques including immunohistochemistry (IHC), northern blot analysis (NBA), and reverse transcriptase-polymerase chain reaction (RT-PCR) were employed in parallel to reveal the panendocrine marker chromogranin A (CgA). The data were related to pathological, immunocytochemical (PGP 9.5, synaptophysin, Leu-7 and neuronspecific enolase), and prognostic indicators. Forty surgically resected cases of NSCLC (24 squamous cell carcinomas, 12 ordinary type adenocarcinomas, 3 bronchiolo-alveolar carcinomas, and 1 anaplastic large cell carcinoma), in which fresh frozen material was available for mRNA analysis, were collected. CgA immunoreactivity was present in five cases (12•5 per cent), generally confined to a minority of the neoplastic cell population. By RT-PCR, CgA mRNA was found in 20 cases (50 per cent), including the five tumours positive by IHC. A statistically signifi

Who reads Chromogranin A gene expression in non-small cell lung carcinomas?

Author

Giancarlo Abbona; Mauro Papotti; Laura Viberti; Luigia Macr??; Anna Stella; Gianni Bussolati

Publisher

John Wiley and Sons; Wiley (John Wiley & Sons); John Wiley & Sons Inc.; Wiley (ISSN 0022-3417)

Published

1998

Language

EN

Field

Medicine (Health Sciences)