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Can I read Ca2+-independent but voltage dependent transmission (CiVDS) at a central synapse formed between dorsal root ganglion and dorsal horn neurons on EtoBox?
Ca2+-independent but voltage dependent transmission (CiVDS) at a central synapse formed between dorsal root ganglion and dorsal horn neurons by Yuan Wang; Rong Huang; Zuying Chai; Changhe Wang; Xingyu Du; Yuqi Hang; Jie Li; Xiaohan Jiang; Xi Wu; Zhongjun Qiao; Yinglin Li; Bing Liu; Peng Cao; Feipeng Zhu; Zhuan Zhou is a Biochemistry, Genetics and Molecular Biology article available to read on EtoBox.
What is Ca2+-independent but voltage dependent transmission (CiVDS) at a central synapse formed between dorsal root ganglion and dorsal horn neurons about?
Many studies have shown that alcohol can affect release of neurotransmitter from neurons through pre-and post-synaptic mechanisms. Fewer studies have focused on alcohol's direct effect on fusion of synaptic vesicles, the final step before neurotransmitter release. We tested whether methanol and ethanol affect SNARE driven fusion in a model system of neurotransmitter release. We then identified what parts of our simplified system were specifically affected. Our results show that ethanol is more potent than methanol at increasing fusion probability. The ethanol enhancement is significant at doses that are in the physiologically relevant range. Three different methods showed that alcohol had no effect on SNARE proteins until R10x higher concentrations. Another model of exocytosis, without SNARE proteins (i.e. only lipid membranes), showed an increase in fusion rates under the same conditions. These results provide strong evidence that ethanol alters lipid membranes to enhance fusion in our model of exocytosis.
Who reads Ca2+-independent but voltage dependent transmission (CiVDS) at a central synapse formed between dorsal root ganglion and dorsal horn neurons?
It is typically read by researchers, students, and practitioners in Biochemistry, Genetics and Molecular Biology.
- Author
- Yuan Wang; Rong Huang; Zuying Chai; Changhe Wang; Xingyu Du; Yuqi Hang; Jie Li; Xiaohan Jiang; Xi Wu; Zhongjun Qiao; Yinglin Li; Bing Liu; Peng Cao; Feipeng Zhu; Zhuan Zhou
- Publisher
- Elsevier BV
- Published
- 2023
- Language
- EN
- Field
- Biochemistry, Genetics and Molecular Biology (Life Sciences)