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Functional expression of Kir 6.1/SUR1‐K~ATP~ channels in frog retinal Müller glial cells by Serguei N. Skatchkov; Legier Rojas; Misty J. Eaton; Richard K. Orkand; Bernd Biedermann; Andreas Bringmann; Thomas Pannicke; Rüdiger W. Veh; Andreas Reichenbach is a Neuroscience article available to read on EtoBox.

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## Abstract The retinae and brains of larval and adult amphibians survive long‐lasting anoxia; this finding suggests the presence of functional K~ATP~ channels. We have previously shown with immunocytochemistry studies that retinal glial (Müller) cells in adult frogs express the K~ATP~ channel and receptor proteins, Kir6.1 and SUR1, while retinal neurons display Kir6.2 and SUR2A/B (Skatchkov et al., 2001a: NeuroReport 12:1437–1441; Eaton et al., in press: NeuroReport). Using both immunocytochemistry and electrophysiology, we demonstrate the expression of Kir6.1/SUR1 (K~ATP~) channels in adult frog and tadpole Müller cells. Using conditions favoring the activation of K~ATP~ channels (i.e., ATP‐ and spermine‐free cytoplasm‐dialyzing solution containing gluconate) in Müller cells isolated from both adult frogs and tadpoles, we demonstrate the following. First, using the patch‐clamp technique in whole‐cell recordings, tolbutamide, a blocker of K~ATP~ channels, blocks nearly 100% of the transient and about 30% of the steady‐state inward currents and depolarizes the cell membrane by 5–12 mV. Second, inside‐out membrane patches display a single‐channel inward current induced by gluconate

Who reads Functional expression of Kir 6.1/SUR1‐K~ATP~ channels in frog retinal Müller glial cells?

Author

Serguei N. Skatchkov; Legier Rojas; Misty J. Eaton; Richard K. Orkand; Bernd Biedermann; Andreas Bringmann; Thomas Pannicke; Rüdiger W. Veh; Andreas Reichenbach

Publisher

John Wiley and Sons; Wiley (John Wiley & Sons); John Wiley & Sons Inc.; Wiley; Research Square (ISSN 0894-1491)

Published

2002

Language

EN

Field

Neuroscience (Life Sciences)