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Comparison of transcription mediated amplification (TMA) and reverse transcription polymerase chain reaction (RT-PCR) for detection of hepatitis C virus RNA in liver tissue by Wolf Peter Hofmann; Volker Dries; Eva Herrmann; Barbara Gärtner; Stefan Zeuzem; Christoph Sarrazin is a Medicine article available to read on EtoBox.
What is Comparison of transcription mediated amplification (TMA) and reverse transcription polymerase chain reaction (RT-PCR) for detection of hepatitis C virus RNA in liver tissue about?
## Background: Transcription mediated amplification (tma) is known to be one of the most sensitive detection assays for hepatitis c virus (hcv) rna in serum but has not yet been evaluated in liver tissue. it is unknown whether the higher sensitivity of tma in comparison with polymerase chain reaction (pcr)-based assays is related to a higher efficiency of the extraction and/or amplification step. ## Objectives: The sensitivity of a tma-based assay (versant hcv rna qualitative assay, bayer diagnostics) and a standard rt-pcr-based assay (cobas amplicor hcv 2.0, roche diagnostics) was compared in formalin-fixed paraffin-embedded liver biopsy specimens of patients with chronic hepatitis c. ## Study design: After deparaffinization of 7.5 microm liver sections hcv rna was extracted by standard phenol/chloroform. hcv rna dilution panels were transferred in parallel to cdna synthesis and amplification steps of pcr and tma. furthermore, tma amplification from stepwise diluted hcv sera was performed following rna extraction by either microcentrifuge colums (qiamp viral rna spin kit, qiagen, hilden, germany) or magnetic microparticles (versant hcv rna qualitative assay). ## Results: The total
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- Author
- Wolf Peter Hofmann; Volker Dries; Eva Herrmann; Barbara Gärtner; Stefan Zeuzem; Christoph Sarrazin
- Publisher
- Elsevier BV
- Published
- 2005
- Language
- EN
- Field
- Medicine (Health Sciences)