Can I read CRISPR/Cas9-Based Genome Editing of HSV on EtoBox?

CRISPR/Cas9-Based Genome Editing of HSV by Thilaga Velusamy, Anjali Gowripalan, and David C. Tscharke is a book available to read on EtoBox.

What is CRISPR/Cas9-Based Genome Editing of HSV about?

AbstractThe CRISPR/Cas9 genc editing system is a robust and versatile technology that has revolutionized our capacity for genome cngincering and is applicable in a wide range of organisms, including large dsDNA viruses. Here we provide an efficient methodology that can be used both for marker-based and for marker-free CRISPR/Cas9-mediated editing of the HSV-I genome. In our method, Cas9, guide RNAs and a homology-directed repair template are provided to cells by cotransection of plasmids, followed by intro-duction of the HSV genome by infection. This method offers a great deal of lexibility, facilitating editing of the HSV genome that spans the range from individual nucleotide changes to large deletions and insertions.Key words CRISPR/Cas9, Herpes simplex virus, Genome editing, Recombinant viruses, Homol-ogy-directed repair1 IntroductionThe advent of molecular cloning and genctic cngincering technol-ogies has greatly accelerated basic biological rescarch into herpes simplex viruses (HSV). Until now, a varicty of cmerging technical methods, including RNA interference, gene knockout, and heter-ologous genc expression technologics, have been tailored to inves-tigate HSV pathogenesis a

Author

Thilaga Velusamy, Anjali Gowripalan, and David C. Tscharke

Published

2020

Language

EN