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Nucleoporins facilitate ORC loading onto chromatin by Logan Richards; Christopher L. Lord; Mary Lauren Benton; John A. Capra; Jared T. Nordman is a Biochemistry, Genetics and Molecular Biology article available to read on EtoBox.
What is Nucleoporins facilitate ORC loading onto chromatin about?
## SUMMARY The origin recognition complex (ORC) binds throughout the genome to initiate DNA replication. In metazoans, it is still unclear how ORC is targeted to specific loci to facilitate helicase loading and replication initiation. Here, we perform immunoprecipitations coupled with mass spectrometry for ORC2 in __Drosophila__ embryos. Surprisingly, we find that ORC2 associates with multiple subunits of the Nup107-160 subcomplex of the nuclear pore. Bioinformatic analysis reveals that, relative to all modENCODE factors, nucleoporins are among the most enriched factors at ORC2 binding sites. Critically, depletion of the nucleoporin Elys, a member of the Nup107-160 complex, decreases ORC2 loading onto chromatin. Depleting Elys also sensitizes cells to replication fork stalling, which could reflect a defect in establishing dormant replication origins. Our work reveals a connection between ORC, replication initiation, and nucleoporins, suggesting a function for nucleoporins in metazoan replication initiation.
Who reads Nucleoporins facilitate ORC loading onto chromatin?
It is typically read by researchers, students, and practitioners in Biochemistry, Genetics and Molecular Biology.
- Author
- Logan Richards; Christopher L. Lord; Mary Lauren Benton; John A. Capra; Jared T. Nordman
- Publisher
- Elsevier BV
- Published
- 2022
- Language
- EN
- Field
- Biochemistry, Genetics and Molecular Biology (Life Sciences)