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Loop-mediated Isothermal Amplification-based Nucleic Acid Lateral Flow Assay for the Specific and Multiplex Detection of Genetic Markers by Seokhwan Kim; Jung Ho Kim; Seokjoon Kim; Jung Soo Park; Byung Seok Cha; Eun Sung Lee; Jinjoo Han; Jiye Shin; Youngjun Jang; Ki Soo Park is a Chemistry article available to read on EtoBox.
What is Loop-mediated Isothermal Amplification-based Nucleic Acid Lateral Flow Assay for the Specific and Multiplex Detection of Genetic Markers about?
In this study, a loop-mediated isothermal amplification-based nucleic acid lateral flow assay (LAMP-NALFA) system was developed for the specific and multiplex detection of genetic markers at a low cost. In principle, the LAMP reaction was optimized to generate a single-stranded sequence in the LAMP product, which was designed to serve as a barcode sequence and to specifically bind to the DNA capture on a NALFA strip. As a target genetic marker, the Salmonella enterotoxin (stn) gene was chosen and determined down to 9 aM (∼5.44 copies/μL). Importantly, the proposed system clearly discriminated the specific target amplification products from non-specific amplification products resulting from primers or non-target nucleic acids, proving the high selectivity of the LAMP-NALFA system. Furthermore, the practical applicability of the system was demonstrated by detecting Salmonella bacteria in Luria-Bertani medium, drinking water, and eggshells, with a limit of detection of 1.6 CFU. Finally, two different bacteria (Salmonella and Staphylococcus) were simultaneously determined by the multiplex LAMP-NALFA system. It is expected that the LAMP-NALFA system could be used in a point-of-care sett
Who reads Loop-mediated Isothermal Amplification-based Nucleic Acid Lateral Flow Assay for the Specific and Multiplex Detection of Genetic Markers?
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- Author
- Seokhwan Kim; Jung Ho Kim; Seokjoon Kim; Jung Soo Park; Byung Seok Cha; Eun Sung Lee; Jinjoo Han; Jiye Shin; Youngjun Jang; Ki Soo Park
- Publisher
- Elsevier BV
- Published
- 2022
- Language
- EN
- Field
- Chemistry (Physical Sciences)