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GNAS promotes inflammation-related hepatocellular carcinoma progression by promoting STAT3 activation by Ding, Hongda; Zhang, Xixia; Su, Yang; Jia, Changjun; Dai, Chaoliu is a Biochemistry, Genetics and Molecular Biology article available to read on EtoBox.

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## Background Hepatocellular carcinoma (HCC) is still the most common cause of cancer-related mortality worldwide and accumulating studies report that HCC is frequently linked to chronic inflammation. G-protein alpha-subunit (GNAS)-activating mutations have recently been reported to form a rare subgroup of inflammatory liver tumors. In this study, we investigated the roles of GNAS in inflammation-related HCC progression and its underlying mechanism. ## Methods Lipopolysaccharides (LPS) and diethylnitrosamine were employed to stimulate HCC cells to an induced inflammatory response. qRT-PCR, immunohistochemistry and immunoblotting were performed to detect the expression of GNAS in HCC tissues and cell lines. Expression levels of proinflammatory cytokines were detected by qRT-PCR and ELISA. N6-methyladenosine (m6A) methylation of GNAS mRNA was detected by RNA-binding protein immunoprecipitation (RIP). Transcription factors activation profiling plate array was performed to investigate the underlying mechanism in GNAS promoting interleukin-6 (IL-6) expression in HCC cells. HCC cell invasion was determined by transwell assay in vitro, and tumorigenesis was assessed with a subcutaneous xe

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Author

Ding, Hongda; Zhang, Xixia; Su, Yang; Jia, Changjun; Dai, Chaoliu

Publisher

SP Versita; Springer (Biomed Central Ltd.); BioMed Central Ltd.; Springer Science and Business Media LLC; Walter de Gruyter GmbH (ISSN 1425-8153)

Published

2020

Language

EN

Field

Biochemistry, Genetics and Molecular Biology (Life Sciences)