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The Ionic Products of Bioactive Glass Particle Dissolution Enhance Periodontal Ligament Fibroblast Osteocalcin Expression and Enhance Early Mineralized Tissue Development by Venu G. Varanasi; Jeremy B. Owyoung; Eduardo Saiz; Sally J. Marshall; Grayson W. Marshall; Peter M. Loomer is a Engineering article available to read on EtoBox.

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## Abstract This study resulted in enhanced collagen type 1 and osteocalcin expression in human periodontal ligament fibroblasts (hPDLF) when exposed to bioactive glass conditioned media that subsequently may promote early mineralized tissue development. Commercial BioglassTM (45S5) and experimental bioactive coating glass (6P53‐b), were used to make a glass conditioned media (GCM) for comparison to control medium. ICP‐MS analysis showed increased concentrations of Ca^2+^, PO~4~^3−^, Si^4+^, and Na^+^, for 45S5 GCM and Mg^2+^, K^+^, Ca^2+^, PO~4~^3−^, Si^4+^, and Na^+^ for 6P53‐b GCM (relative to control medium). Differentiating hPDLF cultures exposed to 45S5 and 6P53‐b GCM showed enhanced expression of collagen type 1 (Col1α1, Col1α2), osteocalcin, and alkaline phosphatase gene expression. These GCM also enhanced osteocalcin protein expression. After 16 d of culture, 45S5 and 6P53‐b GCM treated cells showed regions of deep red Alizarin staining, indicating increased Ca within their respective extracellular matrices (ECM), while control‐treated cells did not exhibit these features. SEM analysis showed more developed ECM in GCM treated cultures, indicated by multiple tissue layering

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Author

Venu G. Varanasi; Jeremy B. Owyoung; Eduardo Saiz; Sally J. Marshall; Grayson W. Marshall; Peter M. Loomer

Publisher

John Wiley and Sons; Wiley (John Wiley & Sons); John Wiley & Sons Inc.; Wiley (ISSN 1549-3296)

Published

2011

Language

EN

Field

Engineering (Physical Sciences)