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Determination of Sulfonamides in Milk After Precolumn Derivatisation by Micellar Liquid Chromatography by Mónica Ana Raviolo; Maria Rambla-Alegre; Jenifer Clausell-Tormos; Maria-Elisa Capella-Peiró; Samuel Carda-Broch; Josep Esteve-Romero is a Chemistry article available to read on EtoBox.

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A simple method to identify and determine six sulfonamides (sodium sulfacetamide, sulfamethizole, sulfaguanidine, sulfamerazine, sulfathiazole and sulfamethoxazole) in milk by micellar liquid chromatography (MLC) is reported. The assay makes use of a precolumn diazotisation-coupling derivatisation including the formation of an azo dye that can be detected at 490 nm. Furthermore, the use of MLC as an analytical tool allows the direct injection of non-purified samples. The separation was performed with an 80 mM SDS-8.5% propanol eluent at pH 7. Analysis times are below 16 min with a complete resolution. Linearities (r > 0.9999), as well as intra-and inter-day precision (below 2.7%), were studied in the validation of the method. The limits of detection and quantification ranged from approximately 0.72 to 0.94 and 2.4 to 3.1 ng mL -1 , respectively. The detection limit was below the maximum residue limit established by the European Community. Finally, recoveries in spiked milk samples were in the 83-103% range.

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Author

Mónica Ana Raviolo; Maria Rambla-Alegre; Jenifer Clausell-Tormos; Maria-Elisa Capella-Peiró; Samuel Carda-Broch; Josep Esteve-Romero

Publisher

Elsevier Science; Elsevier ; Elsevier BV (ISSN 0003-2670)

Published

2007

Language

EN

Field

Chemistry (Physical Sciences)