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Sam68 Marks the Transcriptionally Active Stages of Spermatogenesis and Modulates Alternative Splicing in Male Germ Cells by Maria Paola Paronetto; Valeria Messina; Marco Barchi; Raffaele Geremia; Stéphane Richard; Claudio Sette is a Biochemistry, Genetics and Molecular Biology article available to read on EtoBox.
What is Sam68 Marks the Transcriptionally Active Stages of Spermatogenesis and Modulates Alternative Splicing in Male Germ Cells about?
Sam68 plays an essential role in mouse spermatogenesis and male fertility. Herein, we report an interaction between Sam68 and the phosphorylated forms of the RNA polymerase II (RNAPII) in meiotic spermatocytes. RNase treatment decreased but did not abolish the interaction, consistently with __in vitro__ binding of RNAPII to the Sam68 carboxyl-terminal region. Sam68 retention in the spermatocyte nucleus was dependent on the integrity of cellular RNAs, suggesting that the protein is recruited to transcriptionally active chromatin. Mouse knockout models characterized by stage-specific arrest of spermatogenesis and staining with the phosphorylated form of RNAPII documented that Sam68 expression is confined to the transcriptionally active stages of spermatogenesis. Furthermore, Sam68 associates with splicing regulators in germ cells and we report that alternative splicing of __Sgce__ exon 8 is regulated in a Sam68-dependent manner during spermatogenesis. RNA and chromatin crosslink immunoprecipitation experiments showed that Sam68 binds __in vivo__ to sequences surrounding the intron 7/exon 8 boundary, thereby affecting the recruitment of the phosphorylated RNAPII and of the general spl
Who reads Sam68 Marks the Transcriptionally Active Stages of Spermatogenesis and Modulates Alternative Splicing in Male Germ Cells?
It is typically read by researchers, students, and practitioners in Biochemistry, Genetics and Molecular Biology.
- Author
- Maria Paola Paronetto; Valeria Messina; Marco Barchi; Raffaele Geremia; Stéphane Richard; Claudio Sette
- Publisher
- Oxford University Press; London: Information Retrieval; Oxford: Oxford University Press; Oxford University Press (OUP) (ISSN 0305-1048)
- Published
- 2011
- Language
- EN
- Field
- Biochemistry, Genetics and Molecular Biology (Life Sciences)