About this Biochemistry, Genetics and Molecular Biology article

Competition for L-glutamate between specialised and versatile Clostridium species by H. J. Laanbroek; A. J. Smit; G. Klein Nulend; H. Veldkamp is a Biochemistry, Genetics and Molecular Biology article available to read on EtoBox.

Clostridium cochlearium could be reproducibly enriched in an L-aspartate- and L-glutamate-limited, anaerobic chemostat inoculated with anaerobic sludge. L-glutamate, L-glutamine and L-histidine were the only fermentable substrates. Less specialised clostridia of the C. tetanomorphum type could only be isolated from batch enrichments with L-glutamate and L-aspartate as energy sources. Competition experiments with C. cochlearium and C. tetanomorphum in a L-glutamate-limited chemostat resulted in the selective elimination of the latter species. Addition of glucose to the medium resulted in coexistence of both species. The molar growth yields for L-glutamate at different dilution rates at 30 degrees C were determined for both species. The maximum specific growth rates on L-glutamate were 0.55 h-1 for C. cochlearium and 0.35 h-1 for C. tetanomorphum.

Author

H. J. Laanbroek; A. J. Smit; G. Klein Nulend; H. Veldkamp

Publisher

Springer; Springer-Verlag; Springer Verlag; Springer Science and Business Media LLC; Archie Comics (ISSN 0302-8933)

Published

1979

Language

EN

Field

Biochemistry, Genetics and Molecular Biology (Life Sciences)